A detailed description and illustrations of the larva of Telebasis digiticollis Calvert, 1902 are provided. A comparison with other larvae of the genus is made. Telebasis digiticollis is distinguished by 1 premental seta, 6–7 setae on the labial palp, a well-developed and convex ligula, 7–8 spiniform setae on the lateral margins of the prementum, and forceps-like male gonapophyses. The larva of T. digiticollis is more like that of T. boomsmae Garrison, 1994 than like other larvae of the genus. Larvae were found living in a lagoon densely covered by aquatic phanerogams such as Eichhornia sp. (Pontederiaceae) and Typha sp. (Typhaceae).